Details of experiments between the fragments Q13574~919-928 and P29475~9-101
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: NOS1
Protein B fragment: 9-101
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.38
Experiment series 2
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: NOS1
Protein B fragment: 9-101
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.22
Normalized holdup BI: 0.22
Immobilized partner concentration in holdup experiment (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.34
Experiment series 3
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: NOS1
Protein B fragment: 9-101
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.19
Normalized holdup BI: 0.21
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.2