Details of experiments between Q13574 and P78352
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Measured dissociation constant (10-6M): 151.39
Standard deviation of dissociation constant (10-6M): 21.64
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 3.82
Experiment series 2
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: DLG4
Protein B fragment: 155-249
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.25
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.83
Experiment series 3
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.11
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.34
Experiment series 4
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: DLG4
Protein B fragment: 62-152
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 6
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: DLG4
Protein B fragment: 155-249
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.39
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.51
Normalized holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.96
Experiment series 6
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: DLG4
Protein B fragment: 62-152
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.32
Holdup BI standard deviation: 0
Normalized holdup BI: 0.39
Normalized holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.72
Experiment series 7
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.08
Normalized holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 13.5
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 4
Measured pKd: 3.84
Experiment series 8
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: DLG4
Protein B fragment: 306-403
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.06
Normalized holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.59
Experiment series 9
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: DLG4
Protein B fragment: 155-249
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.39
Normalized holdup BI: 0.48
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.76
Experiment series 10
Protein A protein: DGKZ
Protein A fragment: 919-928
Protein A site: PBM
Protein A sequence: IQREDQETAV
Protein A construct: biotin-ttds-IQREDQETAV
Protein B protein: DLG4
Protein B fragment: 62-152
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.29
Normalized holdup BI: 0.34
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.5