Details of experiments between the fragments Q13425~112-199 and P22460~604-613
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: SNTB2
Protein A fragment: 112-199
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: KCNA5
Protein B fragment: 604-613
Protein B site: PBM
Protein B sequence: CLDTSRETDL
Protein B construct: biotin-ttds-CLDTSRETDL

Average holdup BI: 0.41
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.91