Details of experiments between the fragments Q13425~112-199 and P0DTC9~410-419
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SNTB2
Protein A fragment: 112-199
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-N
Protein B fragment: 410-419
Protein B site: PBM
Protein B sequence: SMSSADSTQA
Protein B construct: Biotin-(PEG)3-QQSMSSADSTQA
Average holdup BI: -0.05
Holdup BI standard deviation: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.