Details of experiments between Q13425 and Q6ZTQ3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: SNTB2
Protein A fragment: 112-199
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: RASSF6
Protein B fragment: 360-369
Protein B site: PBM
Protein B sequence: KLVIKTETTV
Protein B construct: biotin-ttds-KLVIKTETTV

Average holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 9
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.2