Details of experiments between Q13424 and P11166
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SNTA1
Protein A fragment: 84-171
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC2A1
Protein B fragment: 483-492
Protein B site: PBM
Protein B sequence: FHPLGADSQV
Protein B construct: biotin-ttds-FHPLGADSQV
Average holdup BI: -0
Holdup BI standard deviation: 0.04
Normalized holdup BI: -0
Normalized holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 4
The affinity was below the detection threshold of the assay.