Details of experiments between the fragments Q13009~1582-1591 and Q12959~314-412
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TIAM1
Protein A fragment: 1582-1591
Protein A site: PBM
Protein A sequence: APSRKLNTEI
Protein A construct: Biotin-Ahx-APSRKLNTEI
Protein B protein: DLG1
Protein B fragment: 314-412
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.12
Normalized holdup BI: 0.14
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.97