Details of experiments between Q13009 and P0DTC3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TIAM1
Protein A fragment: 835-935
Protein A site: PDZ
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-3a
Protein B fragment: 266-275
Protein B site: PBM
Protein B sequence: EPTTTTSVPL
Protein B construct: Biotin-(PEG)3-YDEPTTTTSVPL
Average holdup BI: -0.07
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.