Details of experiments between the fragments Q12959~457-570 and Q9ULB1~1468-1477
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLG1
Protein A fragment: 457-570
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: NRXN1
Protein B fragment: 1468-1477
Protein B site: PBM
Protein B sequence: KKNKDKEYYV
Protein B construct: Biotin-Ahx-KKNKDKEYYV
Average holdup BI: -0.05
Normalized holdup BI: -0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.