Details of experiments between the fragments Q12959~314-412 and Q9UBN1~318-327
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLG1
Protein A fragment: 314-412
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: CACNG4
Protein B fragment: 318-327
Protein B site: PBM
Protein B sequence: SMLNRRTTPV
Protein B construct: biotin-ado-ado-SMLNRRTTPV
Average holdup BI: 0.54
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.6
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.98