Details of experiments between the fragments Q12959~314-412 and A6NIM6~570-579
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLG1
Protein A fragment: 314-412
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: -0.06
Immobilized partner concentration in holdup experiment (10-6M): 3.5
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: DLG1
Protein A fragment: 314-412
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: 0.18
Holdup BI standard deviation: 0.03
Normalized holdup BI: 0.2
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.4
Experiment series 3
Protein A protein: DLG1
Protein A fragment: 314-412
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: biotin-ttds-SSIDLWETAL
Average holdup BI: 0.16
Normalized holdup BI: 0.2
Immobilized partner concentration in holdup experiment (10-6M): 11
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 4.38
Experiment series 4
Protein A protein: DLG1
Protein A fragment: 314-412
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SLC15A5
Protein B fragment: 570-579
Protein B site: PBM
Protein B sequence: SSIDLWETAL
Protein B construct: Biotin-ado-ado-SSIDLWETAL
Average holdup BI: 0.21
Normalized holdup BI: 0.23
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.24