Details of experiments between the fragments Q12959~221-314 and Q9BZC7~2426-2435
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLG1
Protein A fragment: 221-314
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCA2
Protein B fragment: 2426-2435
Protein B site: PBM
Protein B sequence: QLSFNTDTLC
Protein B construct: biotin-ado-ado-QLSFNTDTLC
Average holdup BI: 0.1
Normalized holdup BI: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.87