Details of experiments between the fragments Q12959~221-314 and O95477~2252-2261
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLG1
Protein A fragment: 221-314
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCA1
Protein B fragment: 2252-2261
Protein B site: PBM
Protein B sequence: QDEKVKESYV
Protein B construct: Biotin-Ahx-QDEKVKESYV
Average holdup BI: 0.3
Normalized holdup BI: 0.39
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.6
Experiment series 2
Protein A protein: DLG1
Protein A fragment: 221-314
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ABCA1
Protein B fragment: 2252-2261
Protein B site: PBM
Protein B sequence: QDEKVKESYV
Protein B construct: Biotin-ado-ado-QDEKVKESYV
Average holdup BI: 0.35
Normalized holdup BI: 0.41
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.62