Details of experiments between Q12959 and Q8TDW7
Note that the results of all experiments are listed, regardless of the modification states of the fragments.


Experiment series 1

Protein A protein: DLG1
Protein A fragment: 314-412
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: FAT3
Protein B fragment: 4548-4557
Protein B site: PBM
Protein B sequence: FVETQHQTQV
Protein B construct: biotin-ttds-FVETQHQTQV

Average holdup BI: 0.52
Normalized holdup BI: 0.58
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.95

Experiment series 2

Protein A protein: DLG1
Protein A fragment: 221-314
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: FAT3
Protein B fragment: 4548-4557
Protein B site: PBM
Protein B sequence: FVETQHQTQV
Protein B construct: biotin-ttds-FVETQHQTQV

Average holdup BI: 0.22
Normalized holdup BI: 0.26
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.32

Experiment series 3

Protein A protein: DLG1
Protein A fragment: 457-570
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ

Protein B protein: FAT3
Protein B fragment: 4548-4557
Protein B site: PBM
Protein B sequence: FVETQHQTQV
Protein B construct: biotin-ttds-FVETQHQTQV

Average holdup BI: 0.11
Normalized holdup BI: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.95