Details of experiments between Q12959 and O43182
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLG1
Protein A fragment: 314-412
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARHGAP6
Protein B fragment: 965-974
Protein B site: PBM
Protein B sequence: NPDALPETLV
Protein B construct: biotin-ttds-NPDALPETLV
Average holdup BI: 0.31
Normalized holdup BI: 0.35
Immobilized partner concentration in holdup experiment (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.49
Experiment series 2
Protein A protein: DLG1
Protein A fragment: 221-314
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARHGAP6
Protein B fragment: 965-974
Protein B site: PBM
Protein B sequence: NPDALPETLV
Protein B construct: biotin-ttds-NPDALPETLV
Average holdup BI: 0.26
Normalized holdup BI: 0.3
Immobilized partner concentration in holdup experiment (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.39
Experiment series 3
Protein A protein: DLG1
Protein A fragment: 457-570
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ARHGAP6
Protein B fragment: 965-974
Protein B site: PBM
Protein B sequence: NPDALPETLV
Protein B construct: biotin-ttds-NPDALPETLV
Average holdup BI: 0.05
Normalized holdup BI: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 18.9
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.5