Details of experiments between Q12923 and P23634
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PTPN13
Protein A fragment: 1362-1455
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ATP2B4
Protein B fragment: 1232-1241
Protein B site: PBM
Protein B sequence: SSLQSLETSV
Protein B construct: biotin-ttds-SSLQSLETSV
Average holdup BI: 0.15
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.45
Experiment series 2
Protein A protein: PTPN13
Protein A fragment: 1085-1188
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ATP2B4
Protein B fragment: 1232-1241
Protein B site: PBM
Protein B sequence: SSLQSLETSV
Protein B construct: biotin-ttds-SSLQSLETSV
Average holdup BI: -0.15
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: PTPN13
Protein A fragment: 1495-1591
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ATP2B4
Protein B fragment: 1232-1241
Protein B site: PBM
Protein B sequence: SSLQSLETSV
Protein B construct: biotin-ttds-SSLQSLETSV
Average holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: PTPN13
Protein A fragment: 1779-1873
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ATP2B4
Protein B fragment: 1232-1241
Protein B site: PBM
Protein B sequence: SSLQSLETSV
Protein B construct: biotin-ttds-SSLQSLETSV
Average holdup BI: -0.02
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: PTPN13
Protein A fragment: 1875-1973
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ATP2B4
Protein B fragment: 1232-1241
Protein B site: PBM
Protein B sequence: SSLQSLETSV
Protein B construct: biotin-ttds-SSLQSLETSV
Average holdup BI: -0.05
Immobilized partner concentration in holdup experiment (10-6M): 7
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 6
Protein A protein: PTPN13
Protein A fragment: 1085-1188
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ATP2B4
Protein B fragment: 1232-1241
Protein B site: PBM
Protein B sequence: SSLQSLETSV
Protein B construct: biotin-ttds-SSLQSLETSV
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.5
Experiment series 7
Protein A protein: PTPN13
Protein A fragment: 1085-1188
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: ATP2B4
Protein B fragment: 1232-1241
Protein B site: PBM
Protein B sequence: SSLQSLETSV
Protein B construct: biotin-ttds-SSLQSLETSV
Average holdup BI: 0
Normalized holdup BI: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.