Details of experiments between Q12923 and P0DTC3
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PTPN13
Protein A fragment: 1085-1188
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-3a
Protein B fragment: 266-275
Protein B site: PBM
Protein B sequence: EPTTTTSVPL
Protein B construct: Biotin-(PEG)3-YDEPTTTTSVPL
Average holdup BI: -0.03
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: PTPN13
Protein A fragment: 1362-1455
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-3a
Protein B fragment: 266-275
Protein B site: PBM
Protein B sequence: EPTTTTSVPL
Protein B construct: Biotin-(PEG)3-YDEPTTTTSVPL
Average holdup BI: -0.06
Holdup BI standard deviation: 0.17
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: PTPN13
Protein A fragment: 1495-1591
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-3a
Protein B fragment: 266-275
Protein B site: PBM
Protein B sequence: EPTTTTSVPL
Protein B construct: Biotin-(PEG)3-YDEPTTTTSVPL
Average holdup BI: -0.05
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 4
Protein A protein: PTPN13
Protein A fragment: 1779-1873
Protein A site: PDZ_4
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-3a
Protein B fragment: 266-275
Protein B site: PBM
Protein B sequence: EPTTTTSVPL
Protein B construct: Biotin-(PEG)3-YDEPTTTTSVPL
Average holdup BI: 0.03
Holdup BI standard deviation: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.
Experiment series 5
Protein A protein: PTPN13
Protein A fragment: 1875-1973
Protein A site: PDZ_5
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-3a
Protein B fragment: 266-275
Protein B site: PBM
Protein B sequence: EPTTTTSVPL
Protein B construct: Biotin-(PEG)3-YDEPTTTTSVPL
Average holdup BI: -0.04
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.