Details of experiments between the fragments Q07157~415-516 and Q9IR59~142-151
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TJP1
Protein A fragment: 415-516
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 10
Experiment method: SAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: TJP1
Protein A fragment: 415-516
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: HPV82-E6
Protein B fragment: 142-151
Protein B site: PBM
Protein B sequence: PPRQRSETQV
Protein B construct: biotin-ttds-PPRQRSETQV
Average holdup BI: -0.07
Normalized holdup BI: -0.07
Immobilized partner concentration in holdup experiment (10-6M): 27.4
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.