Details of experiments between the fragments Q07157~184-264 and P0DTC3~266-275
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TJP1
Protein A fragment: 184-264
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: SARS-Cov2-3a
Protein B fragment: 266-275
Protein B site: PBM
Protein B sequence: EPTTTTSVPL
Protein B construct: Biotin-(PEG)3-YDEPTTTTSVPL
Average holdup BI: 0.4
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.56