Details of experiments between the fragments Q07157~184-264 and O15551~211-220
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: TJP1
Protein A fragment: 184-264
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: CLDN3
Protein B fragment: 211-220
Protein B site: PBM
Protein B sequence: GTGYDRKDYV
Protein B construct: biotin-ado-ado-GTGYDRKDYV
Average holdup BI: 0.05
Normalized holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.6