Details of experiments between Q07002 and Q92796
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: CDK18
Protein A fragment: 465-474
Protein A site: PBM
Protein A sequence: RGKNRRQSIF
Protein A construct: biotin-ado-ado-RGKNRRQSIF
Protein B protein: DLG3
Protein B fragment: 221-314
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.2
Normalized holdup BI: 0.21
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.19
Experiment series 2
Protein A protein: CDK18
Protein A fragment: 465-474
Protein A site: PBM
Protein A sequence: RGKNRRQSIF
Protein A construct: biotin-ado-ado-RGKNRRQSIF
Protein B protein: DLG3
Protein B fragment: 381-476
Protein B site: PDZ_3
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.07
Normalized holdup BI: 0.08
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.71
Experiment series 3
Protein A protein: CDK18
Protein A fragment: 465-474
Protein A site: PBM
Protein A sequence: RGKNRRQSIF
Protein A construct: biotin-ado-ado-RGKNRRQSIF
Protein B protein: DLG3
Protein B fragment: 124-219
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.03
Normalized holdup BI: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality causing a systematic error in reported affinities.
PUBMED ID of publication: 36115835
Number of measurements: 1
The affinity was below the detection threshold of the assay.