Details of experiments between the fragments Q04917~1-246 and Q9UQC2~380-389
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAH
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3eta
Protein B protein: GAB2
Protein B fragment: 380-389
Protein B site: 14-3-3_pThr385
Protein B modification: (TPO)385
Protein B sequence: RSVAATIPRR
Protein B construct: RSVAA(pThr)IPRR (crude synthetic peptide)
Average holdup BI: 0.73
Holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 6.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 4.31