Details of experiments between the fragments Q04917~1-246 and Q9UJF2~936-945
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAH
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3eta
Protein B protein: RASAL2
Protein B fragment: 936-945
Protein B site: 14-3-3_pThr941
Protein B modification: (TPO)941
Protein B sequence: IQRQQTQQVQ
Protein B construct: IQRQQ(pThr)QQVQ (crude synthetic peptide)
Average holdup BI: 0.05
Holdup BI standard deviation: 0.22
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.18
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.