Details of experiments between the fragments Q04917~1-246 and Q9H6H4~219-228
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAH
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3eta
Protein B protein: REEP4
Protein B fragment: 219-228
Protein B site: 14-3-3_pSer224
Protein B modification: (SEP)224
Protein B sequence: LIRSQSLRVV
Protein B construct: LIRSQ(pSer)LRVV (crude synthetic peptide)
Average holdup BI: 0.88
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 8.3
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 4.75