Details of experiments between the fragments Q04917~1-246 and Q86X27~417-426
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAH
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3eta
Protein B protein: RALGPS2
Protein B fragment: 417-426
Protein B site: 14-3-3_pSer422
Protein B modification: (SEP)422
Protein B sequence: NRLYHSLGPV
Protein B construct: NRLYH(pSer)LGPV (crude synthetic peptide)
Average holdup BI: 0.89
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 6.57
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 4.79