Details of experiments between the fragments Q04917~1-246 and Q5VV41~102-111
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAH
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3eta
Protein B protein: ARHGEF16
Protein B fragment: 102-111
Protein B site: 14-3-3_pSer107
Protein B modification: (SEP)107
Protein B sequence: PARHQSFGAA
Protein B construct: PARHQ(pSer)FGAA (crude synthetic peptide)
Average holdup BI: -0.21
Holdup BI standard deviation: 0.49
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.42
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.