Details of experiments between the fragments Q04917~1-246 and O96013~202-211
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAH
Protein A fragment: 1-246
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3eta
Protein B protein: PAK4
Protein B fragment: 202-211
Protein B site: 14-3-3_pThr207
Protein B modification: (TPO)207
Protein B sequence: GRPFNTYPRA
Protein B construct: GRPFN(pThr)YPRA (crude synthetic peptide)
Average holdup BI: 0.57
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 130
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 2.65
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 4.01