Details of experiments between P98088 and P49418
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: MUC5AC
Protein A fragment: 1311-1325
Protein A site: PxxP_1
Protein A sequence: IERRVYPCSPTTPVP
Protein A construct: Biotinylated 15-mer peptide (crude)

Protein B protein: AMPH
Protein B fragment: 615-695
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: -0
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
The affinity was below the detection threshold of the assay.


Experiment series 2

Protein A protein: MUC5AC
Protein A fragment: 1563-1577
Protein A site: PxxP_2
Protein A sequence: VSSKPTPTEPSTSSC
Protein A construct: Biotinylated 15-mer peptide (crude)

Protein B protein: AMPH
Protein B fragment: 615-695
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: 0.02
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
The affinity was below the detection threshold of the assay.


Experiment series 3

Protein A protein: MUC5AC
Protein A fragment: 1936-1950
Protein A site: PxxP_3
Protein A sequence: VSSKPTPTEPSTSSC
Protein A construct: Biotinylated 15-mer peptide (crude)

Protein B protein: AMPH
Protein B fragment: 615-695
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: -0.01
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
The affinity was below the detection threshold of the assay.


Experiment series 4

Protein A protein: MUC5AC
Protein A fragment: 5643-5657
Protein A site: PxxP_4
Protein A sequence: SWERGVPVSPMH---
Protein A construct: Biotinylated 15-mer peptide (crude)

Protein B protein: AMPH
Protein B fragment: 615-695
Protein B site: SH3
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SH3

Average holdup BI: -0.01
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: HU (DAPF)
Date of measurement: 2022.06.29.
Number of measurements: 2
The affinity was below the detection threshold of the assay.