Details of experiments between the fragments P78352~306-403 and Q6UXZ0~253-262
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLG4
Protein A fragment: 306-403
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TMIGD1
Protein B fragment: 253-262
Protein B site: PBM
Protein B sequence: DKDPHSETAL
Protein B construct: biotin-ttds-DKDPHSETAL
Measured dissociation constant (10-6M): 178.57
Standard deviation of dissociation constant (10-6M): 10.91
Experiment method: competitive FP
Details of affinity fitting: Competitive binding equation in ProFit
PUBMED ID of publication: 36115835
Number of measurements: 3
Measured pKd: 3.75
Experiment series 2
Protein A protein: DLG4
Protein A fragment: 306-403
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: TMIGD1
Protein B fragment: 253-262
Protein B site: PBM
Protein B sequence: DKDPHSETAL
Protein B construct: biotin-ttds-DKDPHSETAL
Average holdup BI: 0.02
Holdup BI standard deviation: 0.04
Normalized holdup BI: 0.02
Normalized holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 18.1
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.