Details of experiments between P78352 and Q9BST9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: DLG4
Protein A fragment: 306-403
Protein A site: PDZ_3
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RTKN
Protein B fragment: 554-563
Protein B site: PBM
Protein B sequence: QPRTWLQSPV
Protein B construct: Biotin-Ahx-QPRTWLQSPV
Average holdup BI: 0.22
Holdup BI standard deviation: 0.01
Normalized holdup BI: 0.28
Normalized holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 4.35
Experiment series 2
Protein A protein: DLG4
Protein A fragment: 155-249
Protein A site: PDZ_2
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RTKN
Protein B fragment: 554-563
Protein B site: PBM
Protein B sequence: QPRTWLQSPV
Protein B construct: Biotin-Ahx-QPRTWLQSPV
Average holdup BI: 0.2
Normalized holdup BI: 0.24
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.27
Experiment series 3
Protein A protein: DLG4
Protein A fragment: 62-152
Protein A site: PDZ_1
Protein A construct: his6-MBP-TEVsite-PDZ
Protein B protein: RTKN
Protein B fragment: 554-563
Protein B site: PBM
Protein B sequence: QPRTWLQSPV
Protein B construct: Biotin-Ahx-QPRTWLQSPV
Average holdup BI: 0.21
Normalized holdup BI: 0.21
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.19