Details of experiments between the fragments P63104~1-245 and Q96PY6~409-418
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAZ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3zeta
Protein B protein: NEK1
Protein B fragment: 409-418
Protein B site: 14-3-3_pSer414
Protein B modification: (SEP)414
Protein B sequence: WRNVLSAGGS
Protein B construct: WRNVL(pSer)AGGS (crude synthetic peptide)
Average holdup BI: 0.34
Holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 132
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 4.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 3.59