Details of experiments between the fragments P63104~1-245 and Q07866~563-572
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAZ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3zeta
Protein B protein: KLC1
Protein B fragment: 563-572
Protein B site: 14-3-3_pSer568
Protein B modification: (SEP)568
Protein B sequence: SKLRASIRRS
Protein B construct: SKLRA(pSer)IRRS (crude synthetic peptide)
Average holdup BI: -0.22
Holdup BI standard deviation: 0.3
Immobilized partner concentration in holdup experiment (10-6M): 132
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.98
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.