Details of experiments between the fragments P63104~1-245 and P27448~559-568
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAZ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3zeta
Protein B protein: MARK3
Protein B fragment: 559-568
Protein B site: 14-3-3_pThr564
Protein B modification: (TPO)564
Protein B sequence: TASRSTFHGQ
Protein B construct: TASRS(pThr)FHGQ (crude synthetic peptide)
Measured dissociation constant (10-6M): <100
Immobilized partner concentration in holdup experiment (10-6M): 132
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 1
Measured pKd: 4