Details of experiments between P63104 and Q9BZF9
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAZ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3zeta
Protein B protein: UACA
Protein B fragment: 1348-1357
Protein B site: 14-3-3_pSer1353
Protein B modification: (SEP)1353
Protein B sequence: PTKRQSQLID
Protein B construct: PTKRQ(pSer)QLID (crude synthetic peptide)
Average holdup BI: -0.09
Holdup BI standard deviation: 0.12
Immobilized partner concentration in holdup experiment (10-6M): 132
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 2
Protein A protein: YWHAZ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3zeta
Protein B protein: UACA
Protein B fragment: 1043-1052
Protein B site: 14-3-3_pThr1048
Protein B modification: (TPO)1048
Protein B sequence: DLRDKTVLIE
Protein B construct: DLRDK(pThr)VLIE (crude synthetic peptide)
Average holdup BI: -0.04
Holdup BI standard deviation: 0.09
Immobilized partner concentration in holdup experiment (10-6M): 132
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.38
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 3
Protein A protein: YWHAZ
Protein A fragment: 1-245
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3zeta
Protein B protein: UACA
Protein B fragment: 621-630
Protein B site: 14-3-3_pSer626
Protein B modification: (SEP)626
Protein B sequence: AKLALSIPAE
Protein B construct: AKLAL(pSer)IPAE (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.2
Immobilized partner concentration in holdup experiment (10-6M): 132
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.13
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.