Details of experiments between P63010 and Q96L92
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: AP2B1
Protein A fragment: 1-937
Protein A construct: endogenous protein (Jurkat)

Protein B protein: SNX27
Protein B fragment: 40-141
Protein B site: PDZ
Protein B construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Average holdup BI: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 10
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.19
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.06.27.
Experimental details: -log10(P Two-sided unpaired T-test (6 vs 6))
PUBMED ID of publication: 36542723
Number of measurements: 6
The affinity was below the detection threshold of the assay.


Experiment series 2

Protein A protein: AP2B1
Protein A fragment: 1-937
Protein A construct: endogenous protein (SH-SY5Y)

Protein B protein: SNX27
Protein B fragment: 40-141
Protein B site: PDZ
Protein B construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Average holdup BI: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 10
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.24
Experiment method: nHU_SH-SY5Y
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.06.27.
Experimental details: -log10(P Two-sided unpaired T-test (6 vs 6))
PUBMED ID of publication: 36542723
Number of measurements: 6
The affinity was below the detection threshold of the assay.


Experiment series 3

Protein A protein: AP2B1
Protein A fragment: 1-937
Protein A construct: endogenous protein (Jurkat)

Protein B protein: SNX27
Protein B fragment: 1-541
Protein B construct: avi(biotin)-his6-MBP-TEVsite-SNX27

Average holdup BI: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 10
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1.71
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.06.27.
Experimental details: -log10(P Two-sided unpaired T-test (6 vs 6))
PUBMED ID of publication: 36542723
Number of measurements: 6
The affinity was below the detection threshold of the assay.


Experiment series 4

Protein A protein: AP2B1
Protein A fragment: 1-937
Protein A construct: endogenous protein (Jurkat)

Protein B protein: SNX27
Protein B fragment: 40-141
Protein B site: PDZ
Protein B construct: avi(biotin)-his6-MBP-TEVsite-PDZ

Average holdup BI: -0.04
Immobilized partner concentration in holdup experiment (10-6M): 10
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.55
Experiment method: nHU_Jurkat
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.08.03.
Experimental details: -log10(P Two-sided unpaired T-test (6 vs 3))
PUBMED ID of publication: 36542723
Number of measurements: 3
The affinity was below the detection threshold of the assay.