Details of experiments between the fragments P62258~1-255 and Q13136~695-704
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAE
Protein A fragment: 1-255
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Protein B protein: PPFIA1
Protein B fragment: 695-704
Protein B site: 14-3-3_pSer700
Protein B modification: (SEP)700
Protein B sequence: PGSGRSTPRR
Protein B construct: PGSGR(pSer)TPRR (crude synthetic peptide)
Average holdup BI: -0.05
Holdup BI standard deviation: 0.94
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.