Details of experiments between the fragments P62258~1-255 and Q12802~2694-2703
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAE
Protein A fragment: 1-255
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Protein B protein: AKAP13
Protein B fragment: 2694-2703
Protein B site: 14-3-3_pSer2699
Protein B modification: (SEP)2699
Protein B sequence: LMRIPSFFPS
Protein B construct: LMRIP(pSer)FFPS (crude synthetic peptide)
Average holdup BI: 0.15
Holdup BI standard deviation: 0.57
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.21
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.