Details of experiments between the fragments P62258~1-255 and Q00537~87-96
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAE
Protein A fragment: 1-255
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Protein B protein: CDK17
Protein B fragment: 87-96
Protein B site: 14-3-3_pSer92
Protein B modification: (SEP)92
Protein B sequence: MPRNGSRLDI
Protein B construct: MPRNG(pSer)RLDI (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.72
Immobilized partner concentration in holdup experiment (10-6M): 116
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.02
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.