Details of experiments between the fragments P62258~1-255 and O43896~669-678
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAE
Protein A fragment: 1-255
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3epsilon
Protein B protein: KIF1C
Protein B fragment: 669-678
Protein B site: 14-3-3_pSer674
Protein B modification: (SEP)674
Protein B sequence: RLYADSDSGD
Protein B construct: RLYAD(pSer)DSGD (crude synthetic peptide)
Average holdup BI: -0.29
Holdup BI standard deviation: 0.7
Immobilized partner concentration in holdup experiment (10-6M): 143
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.58
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.