Details of experiments between the fragments P61981~1-247 and P49761~194-203
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A modification: 100_uM_FCA
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: CLK3
Protein B fragment: 194-203
Protein B site: 14-3-3_pSer199
Protein B modification: (SEP)199
Protein B sequence: RSRSRSHDRL
Protein B construct: RSRSR(pSer)HDRL (crude synthetic peptide)
Average holdup BI: 0.24
Holdup BI standard deviation: 0.33
Immobilized partner concentration in holdup experiment (10-6M): 115
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.34
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.