Details of experiments between the fragments P61981~1-247 and O60343~339-348
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A modification: 100_uM_FCA
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: TBC1D4
Protein B fragment: 339-348
Protein B site: 14-3-3_pSer344
Protein B modification: (SEP)344
Protein B sequence: HASAPSHVQP
Protein B construct: HASAP(pSer)HVQP (crude synthetic peptide)
Average holdup BI: -0.3
Holdup BI standard deviation: 1.14
Immobilized partner concentration in holdup experiment (10-6M): 115
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.26
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.