Details of experiments between the fragments P61981~1-247 and Q12778~251-260
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: FOXO1
Protein B fragment: 251-260
Protein B site: 14-3-3_pSer256
Protein B modification: (SEP)256
Protein B sequence: RRRAASMDNN
Protein B construct: RRRAA(pSer)MDNN (crude synthetic peptide)
Average holdup BI: -0.15
Holdup BI standard deviation: 0.45
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.29
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.