Details of experiments between the fragments P61981~1-247 and P15056~600-609
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: BRAF
Protein B fragment: 600-609
Protein B site: 14-3-3_pSer605
Protein B modification: (SEP)605
Protein B sequence: VKSRWSGSHQ
Protein B construct: VKSRW(pSer)GSHQ (crude synthetic peptide)
Average holdup BI: 0.16
Holdup BI standard deviation: 1.34
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.07
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.