Details of experiments between P61981 and Q13136
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 663-672
Protein B site: 14-3-3_pSer668
Protein B modification: (SEP)668
Protein B sequence: RVGSGSLDNL
Protein B construct: RVGSG(pSer)LDNL (crude synthetic peptide)
Average holdup BI: 0.41
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 9.32
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 3.71
Experiment series 2
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 1154-1163
Protein B site: 14-3-3_pThr1159
Protein B modification: (TPO)1159
Protein B sequence: AGSAETLPAN
Protein B construct: AGSAE(pThr)LPAN (crude synthetic peptide)
Average holdup BI: 0.57
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 129
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 8.42
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 4.02
Experiment series 3
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 663-672
Protein B site: 14-3-3_pSer668
Protein B modification: (SEP)668
Protein B sequence: RVGSGSLDNL
Protein B construct: RVGSG(pSer)LDNL (crude synthetic peptide)
Average holdup BI: 0.44
Holdup BI standard deviation: 0.02
Immobilized partner concentration in holdup experiment (10-6M): 129
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 8.24
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 3.79
Experiment series 4
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A modification: 100_uM_FCA
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 663-672
Protein B site: 14-3-3_pSer668
Protein B modification: (SEP)668
Protein B sequence: RVGSGSLDNL
Protein B construct: RVGSG(pSer)LDNL (crude synthetic peptide)
Average holdup BI: 0.39
Holdup BI standard deviation: 0.04
Immobilized partner concentration in holdup experiment (10-6M): 115
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 5.86
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 3.76
Experiment series 5
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A modification: 100_uM_FCA
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 525-534
Protein B site: 14-3-3_pSer530
Protein B modification: (SEP)530
Protein B sequence: RPHLGSVPDF
Protein B construct: RPHLG(pSer)VPDF (crude synthetic peptide)
Average holdup BI: 0.64
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 115
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 5.83
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 4.21
Experiment series 6
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A modification: 100_uM_FCA
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 1154-1163
Protein B site: 14-3-3_pThr1159
Protein B modification: (TPO)1159
Protein B sequence: AGSAETLPAN
Protein B construct: AGSAE(pThr)LPAN (crude synthetic peptide)
Average holdup BI: 0.58
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 115
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 5.37
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 4.1
Experiment series 7
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 567-576
Protein B site: 14-3-3_pThr572
Protein B modification: (TPO)572
Protein B sequence: PSKVQTLNEQ
Protein B construct: PSKVQ(pThr)LNEQ (crude synthetic peptide)
Average holdup BI: 0.15
Holdup BI standard deviation: 0.03
Immobilized partner concentration in holdup experiment (10-6M): 129
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 4.67
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 8
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 525-534
Protein B site: 14-3-3_pSer530
Protein B modification: (SEP)530
Protein B sequence: RPHLGSVPDF
Protein B construct: RPHLG(pSer)VPDF (crude synthetic peptide)
Average holdup BI: 0.71
Holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 129
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 4.42
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
Measured pKd: 4.28
Experiment series 9
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 567-576
Protein B site: 14-3-3_pThr572
Protein B modification: (TPO)572
Protein B sequence: PSKVQTLNEQ
Protein B construct: PSKVQ(pThr)LNEQ (crude synthetic peptide)
Average holdup BI: 0.14
Holdup BI standard deviation: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 2.79
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 10
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A modification: 100_uM_FCA
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 567-576
Protein B site: 14-3-3_pThr572
Protein B modification: (TPO)572
Protein B sequence: PSKVQTLNEQ
Protein B construct: PSKVQ(pThr)LNEQ (crude synthetic peptide)
Average holdup BI: 0.17
Holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 115
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 2.64
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 11
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 1154-1163
Protein B site: 14-3-3_pThr1159
Protein B modification: (TPO)1159
Protein B sequence: AGSAETLPAN
Protein B construct: AGSAE(pThr)LPAN (crude synthetic peptide)
Average holdup BI: 0.55
Holdup BI standard deviation: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 2.63
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 3.96
Experiment series 12
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 758-767
Protein B site: 14-3-3_pSer763
Protein B modification: (SEP)763
Protein B sequence: ALHTVSHEDI
Protein B construct: ALHTV(pSer)HEDI (crude synthetic peptide)
Average holdup BI: 0.48
Holdup BI standard deviation: 0.23
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 2.04
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 3.83
Experiment series 13
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A modification: 100_uM_FCA
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 801-810
Protein B site: 14-3-3_pSer806
Protein B modification: (SEP)806
Protein B sequence: KGIKSSIGRL
Protein B construct: KGIKS(pSer)IGRL (crude synthetic peptide)
Average holdup BI: 0.12
Holdup BI standard deviation: 0.07
Immobilized partner concentration in holdup experiment (10-6M): 115
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1.86
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 14
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A modification: 100_uM_FCA
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 695-704
Protein B site: 14-3-3_pSer700
Protein B modification: (SEP)700
Protein B sequence: PGSGRSTPRR
Protein B construct: PGSGR(pSer)TPRR (crude synthetic peptide)
Average holdup BI: 0.71
Holdup BI standard deviation: 0.1
Immobilized partner concentration in holdup experiment (10-6M): 115
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1.53
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
Measured pKd: 4.34
Experiment series 15
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A modification: 100_uM_FCA
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 735-744
Protein B site: 14-3-3_pThr740
Protein B modification: (TPO)740
Protein B sequence: TIKCETSPPS
Protein B construct: TIKCE(pThr)SPPS (crude synthetic peptide)
Average holdup BI: -0.5
Holdup BI standard deviation: 0.56
Immobilized partner concentration in holdup experiment (10-6M): 115
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 1
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 16
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 1134-1143
Protein B site: 14-3-3_pSer1139
Protein B modification: (SEP)1139
Protein B sequence: FRRAPSWRKK
Protein B construct: FRRAP(pSer)WRKK (crude synthetic peptide)
Average holdup BI: -0.47
Holdup BI standard deviation: 0.72
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.77
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 17
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A modification: 100_uM_FCA
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 758-767
Protein B site: 14-3-3_pSer763
Protein B modification: (SEP)763
Protein B sequence: ALHTVSHEDI
Protein B construct: ALHTV(pSer)HEDI (crude synthetic peptide)
Average holdup BI: -0.35
Holdup BI standard deviation: 0.56
Immobilized partner concentration in holdup experiment (10-6M): 115
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.72
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 18
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 801-810
Protein B site: 14-3-3_pSer806
Protein B modification: (SEP)806
Protein B sequence: KGIKSSIGRL
Protein B construct: KGIKS(pSer)IGRL (crude synthetic peptide)
Average holdup BI: 0.03
Holdup BI standard deviation: 0.06
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.45
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 19
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 695-704
Protein B site: 14-3-3_pSer700
Protein B modification: (SEP)700
Protein B sequence: PGSGRSTPRR
Protein B construct: PGSGR(pSer)TPRR (crude synthetic peptide)
Average holdup BI: 0.36
Holdup BI standard deviation: 0.64
Immobilized partner concentration in holdup experiment (10-6M): 134
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.27
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 20
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A modification: 100_uM_FCA
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 1134-1143
Protein B site: 14-3-3_pSer1139
Protein B modification: (SEP)1139
Protein B sequence: FRRAPSWRKK
Protein B construct: FRRAP(pSer)WRKK (crude synthetic peptide)
Average holdup BI: 0.11
Holdup BI standard deviation: 0.33
Immobilized partner concentration in holdup experiment (10-6M): 115
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.24
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2022.02.17.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 4))
Number of measurements: 4
The affinity was below the detection threshold of the assay.
Experiment series 21
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 758-767
Protein B site: 14-3-3_pSer763
Protein B modification: (SEP)763
Protein B sequence: ALHTVSHEDI
Protein B construct: ALHTV(pSer)HEDI (crude synthetic peptide)
Average holdup BI: 0.11
Holdup BI standard deviation: 0.49
Immobilized partner concentration in holdup experiment (10-6M): 129
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.17
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 22
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 735-744
Protein B site: 14-3-3_pThr740
Protein B modification: (TPO)740
Protein B sequence: TIKCETSPPS
Protein B construct: TIKCE(pThr)SPPS (crude synthetic peptide)
Average holdup BI: 0.1
Holdup BI standard deviation: 0.87
Immobilized partner concentration in holdup experiment (10-6M): 129
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.09
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 23
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 1134-1143
Protein B site: 14-3-3_pSer1139
Protein B modification: (SEP)1139
Protein B sequence: FRRAPSWRKK
Protein B construct: FRRAP(pSer)WRKK (crude synthetic peptide)
Average holdup BI: 0.04
Holdup BI standard deviation: 0.46
Immobilized partner concentration in holdup experiment (10-6M): 129
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0.07
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 24
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 801-810
Protein B site: 14-3-3_pSer806
Protein B modification: (SEP)806
Protein B sequence: KGIKSSIGRL
Protein B construct: KGIKS(pSer)IGRL (crude synthetic peptide)
Average holdup BI: 0
Holdup BI standard deviation: 0.13
Immobilized partner concentration in holdup experiment (10-6M): 129
P value (usually -log10(P Two-sided unpaired T-test), but double check the experimental details): 0
Experiment method: HU Multiplex
Details of affinity fitting: hyperbolic binding equation
Date of measurement: 2021.10.13.
Experimental details: -log10(P Two-sided unpaired T-test (4 vs 5))
Number of measurements: 5
The affinity was below the detection threshold of the assay.
Experiment series 25
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 663-672
Protein B site: 14-3-3_pSer668
Protein B modification: (SEP)668
Protein B sequence: RVGSGSLDNL
Protein B construct: biotin-ttds-RVGSG(pSer)LDNL (HPLC-purified synthetic peptide)
Measured dissociation constant (10-6M): 255
Standard deviation of dissociation constant (10-6M): 35
Experiment method: competitive Fluorescence Polarization
Details of affinity fitting: Competitive binding equation in ProFit
Date of measurement: 2022.01.18
Number of measurements: 3
Measured pKd: 3.59
Experiment series 26
Protein A protein: YWHAG
Protein A fragment: 1-247
Protein A site: 14-3-3
Protein A construct: avi-his6-MBP-TEVsite-14-3-3gamma
Protein B protein: PPFIA1
Protein B fragment: 663-672
Protein B site: 14-3-3_pSer668
Protein B modification: (SEP)668
Protein B sequence: RVGSGSLDNL
Protein B construct: biotin-ttds-RVGSG(pSer)LDNL (HPLC-purified synthetic peptide)
Measured dissociation constant (10-6M): 125
Standard deviation of dissociation constant (10-6M): 5
Experiment method: competitive Fluorescence Polarization
Details of affinity fitting: Competitive binding equation in ProFit
Date of measurement: 2022.01.10
Number of measurements: 3
Measured pKd: 3.9