Details of experiments between the fragments P60484~394-403 and O14907~7-124
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PTEN
Protein A fragment: 394-403
Protein A site: PBM
Protein A sequence: EDQHTQITKV
Protein A construct: Biotin-ado-ado-EDQHTQITKV
Protein B protein: TX1BP3
Protein B fragment: 7-124
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.05
Normalized holdup BI: 0.05
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: Crude peptide synthesis product. The peptide concentration in the affinity conversion step may differ from reality. Not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 3.49