Details of experiments between the fragments P60484~393-403 and Q02410~743-822
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PTEN
Protein A fragment: 393-403
Protein A site: PBM
Protein A modification: (ALY)402
Protein A sequence: EDQHTQITKV
Protein A construct: biotin-ttds-DEDQHTQITKacV
Protein B protein: APBA1
Protein B fragment: 743-822
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.18
Normalized holdup BI: 0.23
Immobilized partner concentration in holdup experiment (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.26
Experiment series 2
Protein A protein: PTEN
Protein A fragment: 393-403
Protein A site: PBM
Protein A sequence: EDQHTQITKV
Protein A construct: biotin-ttds-DEDQHTQITKV
Protein B protein: APBA1
Protein B fragment: 743-822
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.13
Normalized holdup BI: 0.16
Immobilized partner concentration in holdup experiment (10-6M): 19
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.02
Experiment series 3
Protein A protein: PTEN
Protein A fragment: 393-403
Protein A site: PBM
Protein A modification: (ALY)402
Protein A sequence: EDQHTQITKV
Protein A construct: biotin-ttds-DEDQHTQITKacV
Protein B protein: APBA1
Protein B fragment: 743-822
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.22
Normalized holdup BI: 0.26
Immobilized partner concentration in holdup experiment (10-6M): 17
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #5
Number of measurements: 1
Measured pKd: 4.35
Experiment series 4
Protein A protein: PTEN
Protein A fragment: 393-403
Protein A site: PBM
Protein A modification: (ALY)402
Protein A sequence: EDQHTQITKV
Protein A construct: biotin-ttds-DEDQHTQITKacV
Protein B protein: APBA1
Protein B fragment: 743-822
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.24
Normalized holdup BI: 0.26
Immobilized partner concentration in holdup experiment (10-6M): 18
Experiment method: DAPF_holdup
Details of affinity fitting: fluorescein + mCherry internal standards + normalization based on CALIP_holdup data + reverse layout
Experimental details: not reported internal standards of holdup layout #6
PUBMED ID of publication: 36115835
Number of measurements: 1
Measured pKd: 4.32