Details of experiments between the fragments P60484~391-403 and O75970~998-1105
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: PTEN
Protein A fragment: 391-403
Protein A site: PBM
Protein A sequence: EDQHTQITKV
Protein A construct: Biotin-ttds-PFDEDQHTQITKV

Protein B protein: MPDZ
Protein B fragment: 998-1105
Protein B site: PDZ_6
Protein B construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.03
Immobilized partner concentration in holdup experiment (10-6M): 17
Experiment method: CALIP_holdup
Details of affinity fitting: lysozyme internal standard
PUBMED ID of publication: 33382810
Number of measurements: 1
The affinity was below the detection threshold of the assay.