Details of experiments between the fragments P60484~391-403 and O15018~64-181
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: PTEN
Protein A fragment: 391-403
Protein A site: PBM
Protein A sequence: EDQHTQITKV
Protein A construct: Biotin-ttds-PFDEDQHTQITKV
Protein B protein: PDZD2
Protein B fragment: 64-181
Protein B site: PDZ_1
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: -0.01
Immobilized partner concentration in holdup experiment (10-6M): 17
Experiment method: CALIP_holdup
Details of affinity fitting: lysozyme internal standard
PUBMED ID of publication: 33382810
Number of measurements: 1
The affinity was below the detection threshold of the assay.