Details of experiments between the fragments P59637~67-76 and Q07157~184-264
Note that the results of all experiments are listed, regardless of the modification states of the fragments.
Experiment series 1
Protein A protein: SARS-Cov-E
Protein A fragment: 67-76
Protein A site: PBM
Protein A sequence: SSEGVPDLLV
Protein A construct: Biotin-(PEG)3-NLNSSEGVPDLLV
Protein B protein: TJP1
Protein B fragment: 184-264
Protein B site: PDZ_2
Protein B construct: his6-MBP-TEVsite-PDZ
Average holdup BI: 0.72
Holdup BI standard deviation: 0.01
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
Measured pKd: 5.18