Details of experiments between P59637 and Q5EBL8
Note that the results of all experiments are listed, regardless of the modification states of the fragments.



Experiment series 1

Protein A protein: SARS-Cov-E
Protein A fragment: 67-76
Protein A site: PBM
Protein A sequence: SSEGVPDLLV
Protein A construct: Biotin-(PEG)3-NLNSSEGVPDLLV

Protein B protein: PDZD11
Protein B fragment: 37-128
Protein B site: PDZ
Protein B construct: his6-MBP-TEVsite-PDZ

Average holdup BI: -0.04
Holdup BI standard deviation: 0
Immobilized partner concentration in holdup experiment (10-6M): 20
Experiment method: CALIP_holdup
Details of affinity fitting: BSA and lysozyme internal standards + bacterial cell lysate + normal layout
PUBMED ID of publication: 36115835
Number of measurements: 2
The affinity was below the detection threshold of the assay.